Expression of Mycoplasma proteins carrying an affinity tag in M. pneumoniae allows rapid purification and circumvents problems related to the aberrant genetic code.

نویسندگان

  • Sebastian R Schmidl
  • Claudine Hames
  • Jörg Stülke
چکیده

In Mycoplasma pneumoniae and several other mollicutes, the UGA opal codon specifies tryptophan rather than a translation stop. This often makes it difficult to express Mycoplasma proteins in heterologous hosts. In this work, we demonstrate that mollicute proteins can be fused to an affinity tag and be expressed directly in M. pneumoniae. The protein can then be purified by affinity chromatography and be used for biochemical or any other desired analysis.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 73 23  شماره 

صفحات  -

تاریخ انتشار 2007